Fig 1: Fam151aKO/KO mice were aged to one year to observe whether they exhibit late onset retinal degeneration not picked up on at the 15 week fundal examination by IMPC. (a) We used CRISPR Cas-9 to produce the Fam151aKO/KO mice and here we show the two separate deletion caused in exon 1 of the gene (bottom line) compared to wild type sequence (top line). (b) Western blot showing loss of Fam151a protein in kidney samples of Fam151aKO/KO mice compared to Fam151a+/+, band at 66 kDa highlighted. Alpha-tubulin used as a loading control. Functional analysis by ERG (c) shows comparable response to a light stimulus as that seen in wild-type littermates. (d) Histology and (e) fundal imaging both show normal retinal morphology, no decrease in photoreceptor layer thickness was seen and fundal images showed an even and healthy appearance. (f) GFAP staining showed no upregulation, indicative of no retinal stress. (g) Comparison of heart weight in relation to body weight between Fam151bKO/KO mutant mice and wild-type littermates. No significant difference was found by t-test (p = 0.1275). Scale bar = 50 µm.